Tianlong Human Influenza Virus B and A Multiplex PCR Kit: Procurement Checks

Human Influenza Virus B (Yamagata& Victoria) and A (H1&H3) Nucleic Acid Multiplex Detection Kit

The Tianlong kit is a fluorescence PCR assay for detecting and differentiating influenza A H1 and H3 and influenza B Yamagata and Victoria lineages from oropharyngeal or nasopharyngeal swabs. The input lists professional laboratories, hospitals, diagnostic centers, and research facilities as intended users.

The supplier states a single-tube format combining reaction solution, primers, and PCR enzymes. The stated PCR detection time is within 40 minutes, though total turnaround also includes extraction, setup, and reporting.

Two catalog configurations are listed: P255H at 25 tests per kit and P655H at 50 tests per kit. Both specify 500 copies/mL sensitivity and precision ≤5.0%, with storage at -25°C to -15°C for 12 months.

Instrument compatibility is stated for FAM, VIC (HEX), ROX (Texas Red), and Cy5 channels, with examples including Applied Biosystems 7500 and Tianlong Gentier systems. Labs should confirm channel calibration and analysis settings before routine use.

The kit carries a CE mark. Procurement should request the current certificate, instructions for use, control requirements, and any destination-market registration documents. Extraction method, transport media, and cold-chain capacity also require verification.

For labs with compatible real-time PCR instruments, the operational case rests on consolidating four targets into one reaction and reducing sample-addition steps. That benefit depends on local validation, specimen performance, and reporting rules.

Why it matters:
A multiplex kit can streamline respiratory testing, but catalog number, instrument channels, cold-chain capacity, and documentation must match actual lab conditions before purchase.

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