BIOER BioRT One-step RT-qPCR All-in-one Master Mix(UDG plus) — Estimated US$220 / 100T

This kit is a single-tube one-step master mix for RNA and DNA nucleic acid detection, optimized for high-throughput workflows. It integrates reverse transcriptase and antibody-modified hot-start Taq DNA polymerase to enhance amplification sensitivity and specificity.

Product details

This kit is a single-tube one-step master mix for RNA and DNA nucleic acid detection, optimized for high-throughput workflows. It integrates reverse transcriptase and antibody-modified hot-start Taq DNA polymerase to enhance amplification sensitivity and specificity. The product incorporates a dUTP/UDG carryover prevention system. The heat-labile UDG functions at room temperature and becomes inactivated during reverse transcription at 50 °C, which effectively minimizes the risk of amplicon aerosol contamination without interfering with the amplification reaction.

Features

𝐑𝐞𝐚𝐝𝐲-𝐭𝐨-𝐔𝐬𝐞 𝐅𝐨𝐫𝐦𝐮𝐥𝐚𝐭𝐢𝐨𝐧: A pre-mixed 2× one-step RT-qPCR master mix. Simply add primers, probe, and template to prepare the reaction.

𝐂𝐚𝐫𝐫𝐲-𝐎𝐯𝐞𝐫 𝐂𝐨𝐧𝐭𝐚𝐦𝐢𝐧𝐚𝐭𝐢𝐨𝐧 𝐂𝐨𝐧𝐭𝐫𝐨𝐥: Heat-labile UDG helps prevent carry-over contamination from previous PCR amplification products.

𝐇𝐢𝐠𝐡 𝐒𝐞𝐧𝐬𝐢𝐭𝐢𝐯𝐢𝐭𝐲 𝐚𝐧𝐝 𝐒𝐩𝐞𝐜𝐢𝐟𝐢𝐜𝐢𝐭𝐲: The integrated reverse transcriptase and antibody-modified hot-start Taq DNA polymerase support sensitive and specific amplification of RNA and DNA targets.

𝐒𝐢𝐦𝐩𝐥𝐢𝐟𝐢𝐞𝐝 𝐎𝐧𝐞-𝐒𝐭𝐞𝐩 𝐖𝐨𝐫𝐤𝐟𝐥𝐨𝐰: Reverse transcription and PCR amplification are performed in a single tube, streamlining reaction setup and supporting high-throughput testing.

Storage and handling

1. When transported at a temperature ≤8 °C for up to 7 days, the product shelf life will not be affected. 2. Kit Storage: Store protected from light at -20 °C. Repeated freeze-thaw cycles should not exceed 5 times. 3. Store protected from light at -20 ± 5°C, shelf life is 12 months.

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