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BIOER Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR) — Estimated US$422.4 / 48T
$422.40
Available in stock
Vibrio cholerae is a gram-negative, comma-shaped bacterium that is the causative agent of cholera, a severe diarrheal illness.
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Scientific China
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| Brand | BIOER |
|---|---|
| Sensitivity | 500 copies/mL |
| Precision | The coefficients of variation for inter-batch, intra-batch, inter-day, and intra-day precision are all less than 5%. |
| Accuracy | Testing with positive reference samples from the company shows a positive agreement rate of 100%, and testing with negative reference samples shows a negative agreement rate of 100%. |
| Specificity | This kit shows no cross-reactivity with Shigella spp., Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Salmonella typhimurium, norovirus, rotavirus, adenovirus, Klebsiella pneumoniae, human genomic DNA, and others. |
| Compatible Platform | FQD-96A, FQD-96C |
| Time | 50 min |
| Recommended Extraction Kit | BSC71 MagaBio Plus Virus DNA/RNA Purification Kit II |
| BSJ39M1 | Product Name: Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR); Packing size: 48T |
| BSJ39L1 | Product Name: Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR); Packing size: 96T |
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Description
Vibrio cholerae is a gram-negative, comma-shaped bacterium that is the causative agent of cholera, a severe diarrheal illness. This pathogen primarily thrives in aquatic environments such as brackish water and estuaries. V. cholerae is categorized into over 200 serogroups, but only O1 and O139 are known to cause cholera outbreaks. The bacterium produces cholera toxin (CT), which disrupts the normal ion transport in the intestines, leading to the characteristic watery diarrhea, also known as "rice-water stool."
Product details
Vibrio cholerae is a gram-negative bacterium that causes cholera, a severe diarrheal disease characterized by sudden onset of profuse, watery diarrhea, which can lead to dehydration and death if untreated. The primary mode of transmission is through contaminated water and food, making the disease particularly prevalent in areas with inadequate sanitation. This Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR) provides a highly sensitive and specific method for detecting the presence of V. cholerae in stool samples. Unlike traditional culture methods, qPCR allows for rapid, qualitative detection, offering results in a much shorter time frame.
Features
High Throughput and Broad Range Detection: The kit offers high detection throughput, capable of simultaneously testing up to 94 samples in a single 96-well experiment.
Rapid Detection: The entire detection process can be completed within 50 minutes.
Contamination-Free Operation: Fully enclosed tube operation eliminates the risk of contamination.
Sample Monitoring: A non-competitive human internal control is included to evaluate sample quality, identify PCR inhibitors, and prevent false-negative results.
Applications
Food and Water Safety Research – Environmental Monitoring Research – Epidemiological Research – Public Health Research – Vibrio cholerae Detection Research
Storage and handling
1. The kit should be stored at -25°C ~ -15°C away from light, and avoid repeated freeze-thaw.
2. The kit can be stored for 5 days at 2-8 °C after opening.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.
4. The kit can be transported in foam box sealed with ice bags or dry ice under 8 ℃.
Specifications
| Sample Type | Stool |
|---|---|
| Sensitivity | 500 copies/mL |
| Precision | The coefficients of variation for inter-batch, intra-batch, inter-day, and intra-day precision are all less than 5%. |
| Accuracy | Testing with positive reference samples from the company shows a positive agreement rate of 100%, and testing with negative reference samples shows a negative agreement rate of 100%. |
| Specificity | This kit shows no cross-reactivity with Shigella spp., Vibrio parahaemolyticus, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Salmonella typhimurium, norovirus, rotavirus, adenovirus, Klebsiella pneumoniae, human genomic DNA, and others. |
| Compatible Platform | FQD-96A, FQD-96C |
| Time | 50 min |
| Recommended Extraction Kit | BSC71 MagaBio Plus Virus DNA/RNA Purification Kit II |
| Certificate | RUO |
Ordering information
| Cat# | Product Name | Packing size |
|---|---|---|
| BSJ39M1 | Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR) | 48T |
| BSJ39L1 | Vibrio Cholerae Nucleic Acid Detection Kit (Fluorescence PCR) | 96T |
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